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HelpTest Code NAIFA Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum
Useful For
Evaluation of patients suspected of having systemic autoimmune rheumatic disease (ANA-associated rheumatic diseases or connective tissue disease) or organ-specific autoimmune diseases such as autoimmune liver diseases
Method Name
Indirect Immunofluorescence
Reporting Name
Antinuclear Ab, HEp-2 Substrate, SSpecimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
<1:80 (Negative)
Day(s) Performed
Monday through Saturday
Report Available
2 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86039
Forms
If not ordering electronically, complete, print, and send General Test Request (T239) with the specimen.
Testing Algorithm
For more information see Connective Tissue Disease Cascade.