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Test Code LAB9360 Perforin in NK and CD8 T Cells

Performing Section

Immunology/Flow

Purpose/Principle

Measurement of intercellular perforin in natural killer cells and CD8+ T cells by flow cytometry analysis.

Specimen Type

Whole Blood:
Purple Top -EDTA (Preferred)
Dark Green Top-Sodium Heparin

Specimen Volume

Preferred: 3.0 mL
Minimum: 0.5 mL

Processing Instructions

Submit whole blood -DO NOT CENTRIFUGE.
Store and send ambient.
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, Sunday or holidays.

Temperature

Ambient

Test Components

Natural Killer Cells
Perforin Positive NK Cells
Perforin Expression in NK Cells
CD8 T Cells
Perforin Positive CD8 T Cells
Perforin Expression in CD8 T Cells

Reference Range

Natural Killer Cells

0 up to 3 months: 4.0-18.0% of lymphocytes

3 months up to 6 months: 3.0-14.0% of lymphocytes

6 months up to 2 years: 3.0-15.0% of lymphocytes

2 years up to 12 years: 4.0-17.0% of lymphocytes

12 years up to 18 years: 3.0-22.0% of lymphocytes

18 years and up: 6.0-27.0% of lymphocytes

 

Perforin Positive NK Cells 0 up to 18 years: >84.0% of NK cells 18 years and up: >93.0% of NK cells

 

Perforin Expression in NK Cells

0 up to 18 years: >1,459.0 Mean fluorescence intensity

18 years and up: >5,207.0 Mean fluorescence intensity

 

CD8 T Cells

0 up to 3 months: 12.0-28.0% of lymphocytes

3 months up to 6 months: 12.0-23.0% of lymphocytes

6 months up to 1 year: 12.0-24.0% of lymphocytes

1 year up to 2 years: 14.0-30.0% of lymphocytes

2 years up to 6 years: 16.0-30.0% of lymphocytes

6 years up to 18 years: 18.0-35.0% of lymphocytes

18 years and up: 12.0-38.0% of lymphocytes

 

Perforin Positive CD8 T Cells

0 up to 18 years: >1.0% of CD8 T cells

18 years and up: >15.0% of CD8 T cells

 

Perforin Expression in CD8 T Cells

0 up to 18 years: >391.0 Mean fluorescence intensity

18 years and up: >763.0 Mean fluorescence intensity

TAT

72 Hours

Sample Stability

Ambient: 48 hours

Additional Notes

Specimen will be rejected if clotted.

CPT

88184
88185 X 5

Test/Batch Schedule

Monday-Friday Noon

Method

Flow Cytometry