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Test Code LAB9360 Perforin in NK and CD8 T Cells

Specimen Type

Whole Blood:
Purple Top -EDTA (Preferred)
Dark Green Top-Sodium Heparin

Processing Instructions

Submit whole blood -DO NOT CENTRIFUGE.
Store and send ambient.
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, Sunday or holidays.

Specimen Volume

Preferred: 3.0 mL
Minimum: 0.5 mL

Temperature

Ambient

Sample Stability

Ambient: 48 hours

Additional Notes

Specimen will be rejected if clotted.

Test/Batch Schedule

Monday-Friday Noon

TAT

72 Hours

Method

Flow Cytometry

Test Components

Natural Killer Cells
Perforin Positive NK Cells
Perforin Expression in NK Cells
CD8 T Cells
Perforin Positive CD8 T Cells
Perforin Expression in CD8 T Cells

Reference Range

Natural Killer Cells

0 up to 3 months: 4.0-18.0% of lymphocytes

3 months up to 6 months: 3.0-14.0% of lymphocytes

6 months up to 2 years: 3.0-15.0% of lymphocytes

2 years up to 12 years: 4.0-17.0% of lymphocytes

12 years up to 18 years: 3.0-22.0% of lymphocytes

18 years and up: 6.0-27.0% of lymphocytes

 

Perforin Positive NK Cells 0 up to 18 years: >84.0% of NK cells 18 years and up: >93.0% of NK cells

 

Perforin Expression in NK Cells

0 up to 18 years: >1, 459.0 Mean fluorescence intensity

18 years and up: >5, 207.0 Mean fluorescence intensity

 

CD8 T Cells

0 up to 3 months: 12.0-28.0% of lymphocytes

3 months up to 6 months: 12.0-23.0% of lymphocytes

6 months up to 1 year: 12.0-24.0% of lymphocytes

1 year up to 2 years: 14.0-30.0% of lymphocytes

2 years up to 6 years: 16.0-30.0% of lymphocytes

6 years up to 18 years: 18.0-35.0% of lymphocytes

18 years and up: 12.0-38.0% of lymphocytes

 

Perforin Positive CD8 T Cells

0 up to 18 years: >1.0% of CD8 T cells

18 years and up: >15.0% of CD8 T cells

 

Perforin Expression in CD8 T Cells

0 up to 18 years: >391.0 Mean fluorescence intensity

18 years and up: >763.0 Mean fluorescence intensity

CPT

88184
88185 X 5

Performing Section

Immunology/Flow

Purpose/Principle

Measurement of intercellular perforin in natural killer cells and CD8+ T cells by flow cytometry analysis.