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Test Code LAB9111 Regulatory T Cells

Performing Section

Immunology/Flow

Purpose/Principle

Flow cytometry analysis of FoxP3 positive Regulatory T cells (Tregs).

Special Collection Notes

No Gel

Specimen Type

Whole Blood:
Purple Top -EDTA (Preferred)
Dark Green Top-Sodium Heparin

Specimen Volume

Preferred: 3.0 mL
Minimum: 1.0 mL

Processing Instructions

Submit whole blood -DO NOT CENTRIFUGE.
Store and send ambient.
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, Sunday or holidays.

Temperature

Ambient

Test Components

Regulatory T Cells (CD4+CD25+FOXP3+)
FOXP3 (Mean Fluorescence Intensity

Reference Range

Regulatory T Cells (CD4+CD25+FOXP3+)

3.2-12.4% of CD4 T Cells

 

FOXP3 (Mean Fluorescence Intensity)

12,872-24,759 Units of Fluorescence

TAT

48 Hours

Sample Stability

Ambient: 24 hours

Additional Notes

Testing must be performed within 24 hours.

 

Specimen will be rejected if clotted.

Aliases

Tregs
Natural Tregs
FOXP3 + T Cell s
FOXP3 mutation
IPEX
T Cell

CPT

88184
88185 X 4

Test/Batch Schedule

Monday-Friday Noon

Method

Flow Cytometry