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HelpTest Code LAB9101 T Cell Proliferation to PHA
Performing Section
Immunology/Flow
Purpose/Principle
Flow cytometry analysis of T cell proliferation following Phytohemagglutinin (PHA) stimulation.
Measurement of T cell proliferation is useful for assessment of immune deficiency and the quality of reconstitution following bone marrow or hematopoietic stem cell transplant. This assay can differentiate between decreased proliferation due to low T cell numbers and due to compromised T cell function.
Special Collection Notes
No Gel
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, or Sunday.
Specimen Type
Whole Blood:
Dark Green Top- Sodium Heparin (Default)
Light Green Top- Lithium Heparin
Specimen Volume
Preferred: 3.0 mL
Minimum: 2.0 mL
Processing Instructions
Submit whole blood -DO NOT CENTRIFUGE.
Store and send ambient.
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, Sunday or holidays.
Temperature
Ambient
Test Components
Sample Viability
CD45+ Lymphocytes PHA 0.3 mcg/mL
CD45+ Lymphocytes PHA 0.6 mcg/mL
CD45+Lymphocytes PHA 1.25 mcg/mL
CD3+T Lymphocytes PHA 0.3 mcg/mL
CD3+T Lymphocytes PHA 0.6 mcg/mL
CD3+T Lymphocytes PHA 1.25 mcg/mL
Reference Range
Sample Viability> 80% of total mononuclear cells
CD45+ Lymphocytes PHA 0.3 mcg/mL
greater than 16% proliferating total lymphocytes
CD45+ Lymphocytes PHA 0.6 mcg/mL
greater than 35.1% proliferating total lymphocytes
CD45+Lymphocytes PHA 1.25 mcg/mL
greater than 49.4% proliferating total lymphocytes
CD3+T Lymphocytes PHA 0.3 mcg/mL
greater than 15.9% of proliferating T cells
CD3+T Lymphocytes PHA 0.6 mcg/mL
greater than 44.1% of proliferating T cells
CD3+T Lymphocytes PHA 1.25 mcg/mL
greater than 60.4% of proliferating T cells
*there is no separate pediatric reference range
TAT
7 days
Sample Stability
Ambient: 48 hours
Additional Notes
Specimen will be rejected if clotted.
Aliases
Phytohemagglutinin (PHA)
Blastogenesis Mitogens
Lymphocyte Transformation
Mitogens
Mitogen Studies
Immune Competence
Lymphocyte Proliferation, Mitogens
T Cell
CPT
88184
88185 X 2
86353 X 3
Test/Batch Schedule
Monday-Friday Noon
Method
Flow Cytometry