Purpose/Principle

This panel includes DNA and RNA analysis of 143 genes that have been described in the diagnosis, prognosis, or treatment decision process for central nervous system tumors.

Detection of single nucleotide variants (SNVs), small indels and flanking intronic regions in the following genes:

ACTB, ACVR1, AKT1, AKT2, AKT3, ALK, APC, AR, ARID1A, ARID1B, ARID2, ATM, ATR, ATRX, AURKA, AURKB, BAP1, BCL2, BCL6, BCOR, BCORL1, BRAF, BRCA1, BRCA2, BRD4, CBL, CCND1, CCND2, CCND3, CD274, CDK4, CDK6, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CHEK2, CIC, CREBBP, CTNNB1, CYSLTR2, DAXX, DDX3X, DICER1, DNMT3A, DROSHA, EGFR, EPHA7, EZH2, FGFR1, FGFR2, FGFR3, FGFR4, FH, FUBP1, GLI1, GLI2, GNA11, GNAQ, GNAS, GPR161, H3F3A, H3F3B, HIST1H1C, HIST1H3B, HIST1H3C, IDH1, IDH2, IKBKAP, JAK2, JAK3, KBTBD4, KDM5A, KDM5C, KDM6A, KIT, KMT2A, KMT2C, KMT2D, KRAS, LZTR1, MAP2K1, MDM2, MDM4, MET, MLH1, MSH2, MSH6, MYB, MYBL1, MYC, MYCN, NF1, NF2, NOTCH1, NOTCH2, NRAS, NTRK1, NTRK2, NTRK3, PALB2, PDCD1, PDGFRA, PDGFRB, PIK3CA, PIK3CB, PIK3CG, PIK3R1, PIK3R2, PLAGL1, PLCB4, PMS2, POLE, POLR2A, PPM1D, PRKAR1A, PRKCA, PTCH1, PTEN, PTPN11, RB1, RET, SDHA, SDHB, SDHC, SDHD, SETD2, SMARCA4, SMARCB1, SMO, STAG2, STAT3, SUFU, TERC, TERT, TET2, TP53, TRAF7, TSC1, TSC2, VHL, WT1, ZMYM3

Detection of gene fusion events within the following genes:

ABI1,ABL1,ABL2,ACACA,ACE,ACER1,ACKR3,ACSL6,ACTB,ADD3,AFF1,AFF3,AFF4,AGR3,AHI1,AHRR,ALK,ANKRD28, AR,ARHGAP20,ARHGAP26,ARNT,ASPSCR1,ASTN2,ATF1,ATIC,ATP1B4,AUTS2,AXL,BACH2,BAG4,BAIAP2L1,BAZ2A,BCAS3, BCAS4,BCL10,BCL11A,BCL11B,BCL2,BCL2L1,BCL3,BCL6,BCL9,BCOR,BCORL1,BCR,BDNF,BICC1,BIRC3,BIRC6,BLNK,BRAF, BRD1,BRD3,BRD4,BRWD3,BTBD18,BTG1,C11orf1,C2CD2L,CAMTA1,CAPRIN1,CARS,CASC5,CASP7,CBFA2T3,CBFB,CBL, CCAR2,CCDC28A,CCDC6,CCDC88C,CCNB1IP1,CCNB3,CCND1,CCND2,CCND3,CD74,CDH11,CDK5RAP2,CDK6,CDX1,CDX2, CEBPA,CEBPB,CEBPD,CEBPE,CEP170B,CEP85L,CHD6,CHIC2,CHMP2B,CHST11,CIC,CIITA,CITED2,CLP1,CLTC,CLTCL1, CMKLR1,CNBP,CNOT2,CNTRL,COG5,COL1A1,COL1A2,COL6A3,COX6C,CPSF6,CRADD,CREB1,CREB3L1,CREB3L2,CREBBP, CRLF1,CRLF2,CRTC1,CSF1,CSF1R,CTDSP2,CTNNB1,CUX1,DAB2IP,DACH1,DACH2,DDIT3,DDX10,DDX20,DEK,DGKH, DMRT1,DNAJB1,DPM1,DUSP22,EBF1,EEFSEC,EGFR,EGR1,EGR2,EGR3,EGR4,EIF4A2,ELF4,ELK4,ELL,ELN,EML1,EML4,EP300, EP400,EPC1,EPOR,EPS15,ERBB2,ERBB3,ERC1,ERCC1,ERG,ERLIN2,ESR1,ETS1,ETV1,ETV4,ETV5,ETV6,EVL,EWSR1,EZR, FAM19A2,FCGR2B,FCRL4,FEN1,FEV,FGF8,FGFR1,FGFR1OP,FGFR1OP2,FGFR2,FGFR3,FGFR4,FGR,FHIT,FIP1L1,FLI1,FLNA, FLT3,FLT3LG,FNBP1,FOS,FOSB,FOSL1,FOXO1,FOXO4,FOXP1,FRK,FRYL,FUS,GAS7,GATA1,GIT2,GLI1,GLIS2,GOSR1,GOT1, GPR128,GPR34,GRHPR,GRID1,GRM1,GTF2I,H2AFX,HAS2,HEY1,HHEX,HIP1,HIPK1,HIST1H4I,HLF,HMGA2,HNF1A, HOXA10,HOXA11,HOXA13,HOXA9,HOXC11,HOXC13,HOXD11,HOXD13,HRAS,HSP90AA1,ID4,IKZF1,IKZF2,IKZF3,IL2, IL21R,IL2RB,IL3,INPP5D,INSR,IQCG,IRF2BP2,IRF4,IRS4,ITK,JAK1,JAK2,JAZF1,KANK1,KAT6A,KAT6B,KDM5A,KIAA1524, KIF5B,KMT2A,KMT2B,KMT2C,KMT2D,KPNB1,KRAS,KSR1,LASP1,LCK,LCP1,LGR5,LHFP,LHX2,LHX4,LMBRD1,LMO1,LMO2, LNP1,LPP,LPXN,LRMP,LRRC37B,LTBP1,LYL1,MACROD1,MAF,MAFB,MALT1,MAML2,MAN2B1,MAPRE1,MBNL1,MBTD1,MDS2, MEAF6,MECOM,MEF2D,MET,MGEA5,MKL1,MKL2,MLF1,MLLT1,MLLT10,MLLT11,MLLT3,MLLT4,MLLT6,MN1,MNX1,MSI2,MSN, MTCP1,MUC1,MUTYH,MYB,MYBL1,MYC,MYH11,MYH9,MYO18A,MYO1F,NAB2,NAPA,NBR1,NCOA1,NCOA2,NCOA3,NCOR1, NDE1,NF1,NFATC2,NFIB,NGF,NGFR,NIN,NIPBL,NKX2-1,NKX2-5,NONO,NOTCH1,NOTCH2,NPM1,NR4A3,NR6A1,NRAS,NRG1, NSD1,NT5C2,NTF3,NTF4,NTRK1,NTRK2,NTRK3,NUMA1,NUMBL,NUP107,NUP214,NUP98,NUTM1,NUTM2A,NUTM2B,OFD1, OLIG2,OLR1,OMD,P2RY8,PAPPA,PATZ1,PAX3,PAX5,PAX7,PAX8,PBX1,PCM1,PDE4DIP,PDGFB,PDGFRA,PDGFRB,PER1,PGR, PHF1,PHF23,PICALM,PIK3CA,PIM1,PKN1,PLAG1,PML,POM121,POU2AF1,POU5F1,PPAP2B,PPARG,PPARGC1A,PPFIBP1, PPP2R1B,PRCC,PRDM16,PRKACA,PRKAR1A,PRKCA,PRKCB,PRKG2,PRRX2,PSIP1,PSMD2,PTK2B,PTPRR,RABEP1,RAD51B, RAF1,RANBP2,RAP1GDS1,RARA,RBM15,RBM6,RCOR1,RCSD1,RELA,RET,RHOH,RNF213,ROS1,RPL22,RPN1,RREB1,RRM1, RSPO2,RSPO3,RTEL1,RUNX1,RUNX1T1,SARNP,SEC31A,SEPTIN2,SEPTIN5,SEPTIN6,SEPTIN9,SERPINE1,SERPINF1,SET, SETBP1,SFPQ,SH3D19,SH3GL1,SIK3,SLC34A2,SLC45A3,SLCO1B3,SMAP1,SMARCA5,SMARCB1,SNHG5,SORBS2,SORT1, SP3,SPECC1,SPI1,SPTBN1,SQSTM1,SRF,SRSF3,SS18,SS18L1,SSBP2,SSX1,SSX2,SSX4,ST6GAL1,STAT5B,STAT6,STIL, STMN1,STRN,SUFU,SUGP2,SUZ12,SYK,TACC1,TACC2,TACC3,TAF15,TAL1,TAL2,TAOK1,TBX15,TCF12,TCF3,TCF7,TCL1A, TCTA,TEAD1,TEAD2,TEAD3,TEAD4,TEC,TENM1,TERT,TET1,TFE3,TFEB,TFG,TFPT,TFRC,TGFBR3,THADA,THRAP3,TIRAP, TLX1,TLX3,TMPRSS2,TNFRSF17,TOP1,TOP2B,TP53BP1,TP63,TP73,TPM3,TPM4,TRA,TRB,TRD,TRHDE,TRIM24,TRIP11, TRPS1,TSLP,TTYH1,TYK2,USP16,USP42,USP6,VGLL2,VGLL3,WASF2,WDR18,WDR70,WHSC1,WHSC1L1,WSB1,WT1, WWTR1,XIAP,YAP1,YTHDF2,YWHAE,ZBTB16,ZC3H7A,ZC3H7B,ZFP64,ZFPM2,ZFTA,ZFYVE19,ZMIZ1,ZMYM2,ZMYND11, ZNF207,ZNF384,ZNF444,ZNF521,ZNF585B,ZNF687