Purpose/Principle

This panel includes DNA and RNA analysis of 184 genes that have been described in the diagnosis, prognosis, or treatment decision process for a variety of solid tumors.  This panel was created with a focus on pediatric solid tumors but may be clinically relevant for a variety of adult neoplasms as well. 

Detection of single nucleotide variants (SNVs), small indels and flanking intronic regions in the following genes:

ABL1,ACTB,ACTL6A,ACTL6B,AKT1,AKT2,AKT3,ALK,AMER1,APC,AR,ARAF,ARID1A,ARID1B,ARID2,ATM,ATR,ATRX,AURKA,AURKB,AXIN1,B2M,BCL2, BCL6,BCOR,BCORL1,BRAF,BRCA1,BRCA2,BRD4,BRIP1,CCND1,CCND2,CCND3,CD274,CDH1,CDK4,CDK6,CDKN1B,CDKN2A,CDKN2B,CDKN2C, CHEK2,CIC,CREBBP,CTNNB1,DAXX,DDR2,DDX3X,DICER1,DLG2,DPF1,DPF2,DPF3,DROSHA,EED,EGFR,EP300,ERBB2,ERBB3,ERG,ESR1,ETV6,EZH2, FGFR1,FGFR2,FGFR3,FGFR4,FH,FLCN,FUBP1,GLI2,GNA11,GNAQ,GNAS,GPC3,H3F3A,H3F3B,HIST1H1C,HIST1H3B,HRAS,IDH1,IDH2,IGF1R,IRS1, JAK1,JAK2,JAK3,JUN,KDM5A,KDM5C,KDM6A,KDR,KIT,KMT2A,KMT2C,KRAS,MAP2K1,MAP2K2,MAP2K4,MAP3K1,MAP3K3,MAPK1,MDM2,MDM4,MECOM, MEN1,MET,MLH1,MSH2,MSH6,MTOR,MYB,MYBL1,MYC,MYCN,MYOD1,NF1,NF2,NOTCH1,NOTCH2,NRAS,NTRK1,NTRK2,NTRK3,PAX5,PBRM1,PDCD1, PDGFRA,PDGFRB,PHF10,PHOX2B,PIK3CA,PIK3CB,PIK3CG,PIK3R1,PIK3R2,PMS2,POLD1,POLE,PPM1D,PTCH1,PTEN,PTPN11,PTPRD,RAF1,RB1,RET, RICTOR,ROS1,RPTOR,SDHA,SDHB,SDHC,SDHD,SETD2,SMAD2,SMAD4,SMARCA2,SMARCA4,SMARCB1,SMARCC1,SMARCC2,SMARCD1,SMARCD2, SMARCD3,SMARCE1,SMO,SRC,STAG2,STAT3,STK11,SUFU,SUZ12,TERC,TERT,TGFBR2,TP53,TP63,TSC1,TSC2,VHL,WT1,XPO1

Detection of gene fusion events within the following genes:

ABI1,ABL1,ABL2,ACACA,ACE,ACER1,ACKR3,ACSL6,ACTB,ADD3,AFF1,AFF3,AFF4,AGR3,AHI1,AHRR,ALK,ANKRD28, AR,ARHGAP20,ARHGAP26,ARNT,ASPSCR1,ASTN2,ATF1,ATIC,ATP1B4,AUTS2,AXL,BACH2,BAG4,BAIAP2L1,BAZ2A,BCAS3, BCAS4,BCL10,BCL11A,BCL11B,BCL2,BCL2L1,BCL3,BCL6,BCL9,BCOR,BCORL1,BCR,BDNF,BICC1,BIRC3,BIRC6,BLNK,BRAF, BRD1,BRD3,BRD4,BRWD3,BTBD18,BTG1,C11orf1,C2CD2L,CAMTA1,CAPRIN1,CARS,CASC5,CASP7,CBFA2T3,CBFB,CBL, CCAR2,CCDC28A,CCDC6,CCDC88C,CCNB1IP1,CCNB3,CCND1,CCND2,CCND3,CD74,CDH11,CDK5RAP2,CDK6,CDX1,CDX2, CEBPA,CEBPB,CEBPD,CEBPE,CEP170B,CEP85L,CHD6,CHIC2,CHMP2B,CHST11,CIC,CIITA,CITED2,CLP1,CLTC,CLTCL1, CMKLR1,CNBP,CNOT2,CNTRL,COG5,COL1A1,COL1A2,COL6A3,COX6C,CPSF6,CRADD,CREB1,CREB3L1,CREB3L2,CREBBP, CRLF1,CRLF2,CRTC1,CSF1,CSF1R,CTDSP2,CTNNB1,CUX1,DAB2IP,DACH1,DACH2,DDIT3,DDX10,DDX20,DEK,DGKH, DMRT1,DNAJB1,DPM1,DUSP22,EBF1,EEFSEC,EGFR,EGR1,EGR2,EGR3,EGR4,EIF4A2,ELF4,ELK4,ELL,ELN,EML1,EML4,EP300, EP400,EPC1,EPOR,EPS15,ERBB2,ERBB3,ERC1,ERCC1,ERG,ERLIN2,ESR1,ETS1,ETV1,ETV4,ETV5,ETV6,EVL,EWSR1,EZR, FAM19A2,FCGR2B,FCRL4,FEN1,FEV,FGF8,FGFR1,FGFR1OP,FGFR1OP2,FGFR2,FGFR3,FGFR4,FGR,FHIT,FIP1L1,FLI1,FLNA, FLT3,FLT3LG,FNBP1,FOS,FOSB,FOSL1,FOXO1,FOXO4,FOXP1,FRK,FRYL,FUS,GAS7,GATA1,GIT2,GLI1,GLIS2,GOSR1,GOT1, GPR128,GPR34,GRHPR,GRID1,GRM1,GTF2I,H2AFX,HAS2,HEY1,HHEX,HIP1,HIPK1,HIST1H4I,HLF,HMGA2,HNF1A, HOXA10,HOXA11,HOXA13,HOXA9,HOXC11,HOXC13,HOXD11,HOXD13,HRAS,HSP90AA1,ID4,IKZF1,IKZF2,IKZF3,IL2, IL21R,IL2RB,IL3,INPP5D,INSR,IQCG,IRF2BP2,IRF4,IRS4,ITK,JAK1,JAK2,JAZF1,KANK1,KAT6A,KAT6B,KDM5A,KIAA1524, KIF5B,KMT2A,KMT2B,KMT2C,KMT2D,KPNB1,KRAS,KSR1,LASP1,LCK,LCP1,LGR5,LHFP,LHX2,LHX4,LMBRD1,LMO1,LMO2, LNP1,LPP,LPXN,LRMP,LRRC37B,LTBP1,LYL1,MACROD1,MAF,MAFB,MALT1,MAML2,MAN2B1,MAPRE1,MBNL1,MBTD1,MDS2, MEAF6,MECOM,MEF2D,MET,MGEA5,MKL1,MKL2,MLF1,MLLT1,MLLT10,MLLT11,MLLT3,MLLT4,MLLT6,MN1,MNX1,MSI2,MSN, MTCP1,MUC1,MUTYH,MYB,MYBL1,MYC,MYH11,MYH9,MYO18A,MYO1F,NAB2,NAPA,NBR1,NCOA1,NCOA2,NCOA3,NCOR1, NDE1,NF1,NFATC2,NFIB,NGF,NGFR,NIN,NIPBL,NKX2-1,NKX2-5,NONO,NOTCH1,NOTCH2,NPM1,NR4A3,NR6A1,NRAS,NRG1, NSD1,NT5C2,NTF3,NTF4,NTRK1,NTRK2,NTRK3,NUMA1,NUMBL,NUP107,NUP214,NUP98,NUTM1,NUTM2A,NUTM2B,OFD1, OLIG2,OLR1,OMD,P2RY8,PAPPA,PATZ1,PAX3,PAX5,PAX7,PAX8,PBX1,PCM1,PDE4DIP,PDGFB,PDGFRA,PDGFRB,PER1,PGR, PHF1,PHF23,PICALM,PIK3CA,PIM1,PKN1,PLAG1,PML,POM121,POU2AF1,POU5F1,PPAP2B,PPARG,PPARGC1A,PPFIBP1, PPP2R1B,PRCC,PRDM16,PRKACA,PRKAR1A,PRKCA,PRKCB,PRKG2,PRRX2,PSIP1,PSMD2,PTK2B,PTPRR,RABEP1,RAD51B, RAF1,RANBP2,RAP1GDS1,RARA,RBM15,RBM6,RCOR1,RCSD1,RELA,RET,RHOH,RNF213,ROS1,RPL22,RPN1,RREB1,RRM1, RSPO2,RSPO3,RTEL1,RUNX1,RUNX1T1,SARNP,SEC31A,SEPTIN2,SEPTIN5,SEPTIN6,SEPTIN9,SERPINE1,SERPINF1,SET, SETBP1,SFPQ,SH3D19,SH3GL1,SIK3,SLC34A2,SLC45A3,SLCO1B3,SMAP1,SMARCA5,SMARCB1,SNHG5,SORBS2,SORT1, SP3,SPECC1,SPI1,SPTBN1,SQSTM1,SRF,SRSF3,SS18,SS18L1,SSBP2,SSX1,SSX2,SSX4,ST6GAL1,STAT5B,STAT6,STIL, STMN1,STRN,SUFU,SUGP2,SUZ12,SYK,TACC1,TACC2,TACC3,TAF15,TAL1,TAL2,TAOK1,TBX15,TCF12,TCF3,TCF7,TCL1A, TCTA,TEAD1,TEAD2,TEAD3,TEAD4,TEC,TENM1,TERT,TET1,TFE3,TFEB,TFG,TFPT,TFRC,TGFBR3,THADA,THRAP3,TIRAP, TLX1,TLX3,TMPRSS2,TNFRSF17,TOP1,TOP2B,TP53BP1,TP63,TP73,TPM3,TPM4,TRA,TRB,TRD,TRHDE,TRIM24,TRIP11, TRPS1,TSLP,TTYH1,TYK2,USP16,USP42,USP6,VGLL2,VGLL3,WASF2,WDR18,WDR70,WHSC1,WHSC1L1,WSB1,WT1, WWTR1,XIAP,YAP1,YTHDF2,YWHAE,ZBTB16,ZC3H7A,ZC3H7B,ZFP64,ZFPM2,ZFTA,ZFYVE19,ZMIZ1,ZMYM2,ZMYND11, ZNF207,ZNF384,ZNF444,ZNF521,ZNF585B,ZNF687