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HelpTest Code LAB7759 PI-Linked Antigen
Performing Section
Immunology/Flow
Purpose/Principle
Flow cytometric analysis of RBC and WBC paroxysmal nocturnal hemoglobinuria (PNH) phenotypes.
Special Collection Notes
Mix sample well after collection to prevent specimen clotting.
Specimen Type
Whole Blood:
Purple Top -EDTA
Specimen Volume
Preferred: 3.0 mL
Minimum: 1.0 mL
Processing Instructions
Submit whole blood -DO NOT CENTRIFUGE.
Store and send ambient.
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, Sunday or holidays.
Temperature
Ambient
Test Components
RBC Type II
RBC Type III
RBC Type II + III
CD14
CD15
CD24
CD45
CD64
FLAER
CD235a
CD59
Reference Range
CD140-100 %
CD15
0-100 %
CD24
0-100 %
CD45
Negative, Dim, Moderate, Bright
CD64
0-100 %
FLAER
0-100 %
CD235a
0-100 %
CD59
0-100 %
TAT
Routine: 48 hours
STAT: 8 hours
Sample Stability
Ambient: 24 hours
Additional Notes
Testing must be completed within 24 hours of collection.
Aliases
PNH
PARAXYSMAL NOCTURNAL HEMOGLOBINURIA
FLAER
CPT
CPT code will vary based on markers identified during processing.
Test/Batch Schedule
Monday-Friday Noon
Method
Flow Cytometry