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Test Code LAB7758 CD3, Peripheral Blood

Performing Section

Immunology/Flow

Purpose/Principle

Testing is performed on peripheral blood collections for CAR-T manufacturing to provide the absolute number of T cells (CD3) per microliter.

Special Collection Notes

Mix sample well after collection to prevent specimen clotting.

Specimen Type

Whole Blood:
Purple Top-EDTA (Preferred)
Dark Green Top-Sodium Heparin
Light Green Top-Lithium Heparin

Specimen Volume

Preferred: 1.0 mL
Minimum: 0.5 mL

Processing Instructions

Submit whole blood -DO NOT CENTRIFUGE.
Store and send ambient.
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, Sunday or holidays.

Temperature

Ambient

Test Components

CD3 Absolute
CD3 Percent

Reference Range

CD3 Absolute

0 up to 3 Months: 2,500-5,500/mcL

3 Months up to 6 Months: 2,500-5,600/mcL

6 Months up to 1 Year: 1,900-5,900/mcL

1 Year up to 2 Years: 2,100-6,200/mcL

2 Years up to 6 Years: 1,400-3,700/mcL

6 Years up to 12 Years: 1,200-2,600/mcL

12 Years up to 18 Years: 1,000-2,200/mcL

8 Years and up: 677-2,383/mcL

 

CD3 Percent

0 up to 3 Months: 53-84%

3 Months up to 6 Months: 51-77%

6 Months up to 1 Year: 49-76%

1 Year up to 2 Years: 53-75%

2 Years up to 6 Years: 56-75%

6 Years up to 12 Years: 60-76%

2 Years up to 18 Years: 56-84%

18 Years and up: 59-83%

TAT

24 hours

Additional Notes

Includes Absolute CD3 and %CD3.

 

CBC with Differential is required for absolute values

Aliases

T Cells

CPT

86359

Test/Batch Schedule

Monday-Friday Noon

Method

Flow Cytometry