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HelpTest Code LAB7758 CD3, Peripheral Blood
Aliases
T Cells
Performing Section
Immunology/Flow
Purpose/Principle
Testing is performed on peripheral blood collections for CAR-T manufacturing to provide the absolute number of T cells (CD3) per microliter.
Special Collection Notes
Mix sample well after collection to prevent specimen clotting.
Specimen Type
Whole Blood:
Purple Top-EDTA (Preferred)
Dark Green Top-Sodium Heparin
Light Green Top-Lithium Heparin
Specimen Volume
Preferred: 1.0 mL
Minimum: 0.5 mL
Processing Instructions
Submit whole blood -DO NOT CENTRIFUGE.
Store and send ambient.
Sample must received into the lab Monday-Friday(before noon). Do not send sample on Friday (after noon), Saturday, Sunday or holidays.
Temperature
Ambient
TAT
24 hours
Test Components
CD3 Absolute
CD3 Percent
CPT
86359
Additional Notes
Includes Absolute CD3 and %CD3.CBC with Differential is required for absolute values
Test/Batch Schedule
Monday-Friday Noon
Method
Flow Cytometry
Reference Range
CD3 Absolute0 up to 3 Months: 2,500-5,500/mcL
3 Months up to 6 Months: 2,500-5,600/mcL
6 Months up to 1 Year: 1,900-5,900/mcL
1 Year up to 2 Years: 2,100-6,200/mcL
2 Years up to 6 Years: 1,400-3,700/mcL
6 Years up to 12 Years: 1,200-2,600/mcL
12 Years up to 18 Years: 1,000-2,200/mcL
8 Years and up: 677-2,383/mcL
CD3 Percent
0 up to 3 Months: 53-84%
3 Months up to 6 Months: 51-77%
6 Months up to 1 Year: 49-76%
1 Year up to 2 Years: 53-75%
2 Years up to 6 Years: 56-75%
6 Years up to 12 Years: 60-76%
2 Years up to 18 Years: 56-84%
18 Years and up: 59-83%