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HelpTest Code LAB7430 Human Herpesvirus 6 PCR Quantitative
Aliases
HHV6 PCR
Special Collection Notes
Collect specimen according to standard operating procedure.
Specimen Type
CSF:
CSF Tube
Sterile screw capped container
Whole Blood:
Purple Top Tube- EDTA (Default)
Processing Instructions
DO NOT CENTRIFUGE.
Store and send ambient STAT.
If transport is longer than 4 hours, send refrigerated same day or overnighted on wet ice.
Specimen Volume
CSF
Preferred: 1.5 mL
Minimum: 1.0 mL
Whole Blood
Preferred: 4.0 mL
Minimum: 0.7 mL
Temperature
Ambient (unless >4 hour then Refrigerated)
Sample Stability
Ambient: 4 hours
Refrigerated: 72 hours
Test/Batch Schedule
Monday – Thursday
TAT
96 Hours
Method
ABI 7500
Test Components
HHV6 PCR Quantitative
HHV6 PCR Quant Value
HHV6 PCR Quant Tissue Value
Reference Range
HHV6 PCR QuantitativeNo HHV6 detected
HHV6 PCR Quant Value
Less than 60 copies/mL
HHV6 PCR Quant Tissue Value
<1 copies per 500ng DNA
CPT
87533
Performing Section
Microbiology
Purpose/Principle
Quantitative HHV-6 DNA PCR can be used to diagnose primary infections, track the course of infection, and monitor response to treatment.
In this assay, DNA is extracted from patient samples and subject to real-time PCR amplification using primers and a dual-labeled fluorescent probe directed against a sequence of the HHV-6 U67 gene. The product is detected using the 5’ – nuclease methodology and quantitated by extrapolation to a standard curve of log10 dilutions of a plasmid containing the target sequence. The assay is linear across six orders of magnitude and can detect as few as 2.5 copies per reaction. An exogenous positive control with its own set of primers and probes is co-amplified in each reaction to detect inhibition.