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HelpTest Code LAB6621 Varicella Zoster Virus PCR
Performing Section
Microbiology
Purpose/Principle
This assay is a real-time PCR system that allows the direct amplification and qualitative detection of VZV DNA from unprocessed specimens without nucleic acid extraction. The system consists of the Simplexa™ VZV Swab Direct assay, the LIAISON® MDX with software, the Direct Amplification Disc (DAD) and accessories. Fluorescent probes are used together with corresponding forward and reverse primers to amplify a well-conserved region of the VZV DNA polymerase gene and internal control targets, used to detect PCR failure or inhibition.
Special Collection Notes
Collect specimen according to standard operating procedure.
Specimen Type
Whole Blood
:Purple Top- EDTA
CSF:
Sterile screw capped container
Standard Swab kit:
cutaneious or mucocutaneous lesions
Specimen Volume
-Whole Blood
Preferred: 3.0 mL
Minimum: 0.8 mL
-CSF
preferred:0.5 mL
Minimum : 0.3 mL
Processing Instructions
Store refrigerated and send sample on wet ice via courier.
If transport time is greater than 24 hours, freeze CSF or plasma at -20° Celsius.
Do not freeze whole blood.
Temperature
Ambient (unless >4 hour then Refrigerated)
Test Components
VZV PCR
Reference Range
Not Detected
TAT
48 Hours
Sample Stability
Ambient: 4 hours
Refrigerated: 72 hours
Additional Notes
Specimen will be rejected if on the incorrect swab or media.
Aliases
VZV PCR
CPT
87798
Test/Batch Schedule
Monday – Sunday
Method
Diasorin Simplexa