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HelpTest Code LAB6621 Varicella Zoster Virus PCR
Aliases
VZV PCR
Special Collection Notes
Collect specimen according to standard operating procedure.
Specimen Type
Whole Blood
:Purple Top- EDTA
CSF:
Sterile screw capped container
Standard Swab kit:
cutaneious or mucocutaneous lesions
Processing Instructions
Store refrigerated and send sample on wet ice via courier.
If transport time is greater than 24 hours, freeze CSF or plasma at -20° Celsius.
Do not freeze whole blood.
Specimen Volume
-Whole Blood
Preferred: 3.0 mL
Minimum: 0.8 mL
-CSF
preferred:0.5 mL
Minimum : 0.3 mL
Temperature
Ambient (unless >4 hour then Refrigerated)
Sample Stability
Ambient: 4 hours
Refrigerated: 72 hours
Additional Notes
Specimen will be rejected if on the incorrect swab or media.
Test/Batch Schedule
Monday – Sunday
TAT
48 Hours
Method
Diasorin Simplexa
Test Components
VZV PCR
Reference Range
Not Detected
CPT
87798
Performing Section
Microbiology
Purpose/Principle
This assay is a real-time PCR system that allows the direct amplification and qualitative detection of VZV DNA from unprocessed specimens without nucleic acid extraction. The system consists of the Simplexa™ VZV Swab Direct assay, the LIAISON® MDX with software, the Direct Amplification Disc (DAD) and accessories. Fluorescent probes are used together with corresponding forward and reverse primers to amplify a well-conserved region of the VZV DNA polymerase gene and internal control targets, used to detect PCR failure or inhibition.