Sign in →

Test Code LAB6500 Chimerism Sorted Cell Analysis, Post-Transplant

Performing Section

PRECISION DIAGNOSTICS

Purpose/Principle

This method uses the Promega PowerPlex® Fusion System for analysis.
Numerous sequence variations have been defined in the human genome which can be used to specifically characterize and distinguish individuals and to define their relatedness within a family or a specific population.

Special Collection Notes

Collect specimen according to standard operating procedure.

Specimen Type

Whole Blood/Bone Marrow : Purple Top Tube-EDTA

 

Mouth: Buccal Swab

 

Tissue (Fresh-Frozen, Paraffin Embedded, or RPMI)

Specimen Volume

Whole Blood/Bone Marrow

Preferred: 4.0 mL

Minimum: 0.5 mL

*Receipt of blood or bone marrow within 72 hours of collection. Delays may impact nucleic acid quality.

 

Tissue

Preferred: uncut paraffin-embedded tissue block. Slides: 1 stained and 10 unstained - 10 micron thick slides

*Receipt within 24 hours of cutting or as soon as possible. Delays may impact nucleic acid quality.

Processing Instructions

Store and ship ambient. If transport is longer than 4 hours send refrigerated on cold pack.
For frozen tissue please send on dry ice- ASAP.

Temperature

Ambient/Refrigerated

Test Components

CD3 Donor Percent
CD3 Donor Result
CD3 Recipient Percent
CD3 Recipient Result
CD33 Donor Percent
CD33 Donor Result
CD33 Recipient Percent
CD33 Recipient Result
CD14 Donor Percent
CD14 Donor Result
CD14 Recipient Percent
CD14 Recipient Result
CD19 Donor Percent
CD19 Donor Result
CD19 Recipient Percent
CD19 Recipient Result
CD56 Donor Percent
CD56 Donor Result
CD56 Recipient Percent
Other Recipient Result
Other Donor Percent
Other Donor Result
Other Recipient Percent
Other Recipient Result

Reference Range

Donor Percent

0-100%

 

Donor Result

Detected, Greater Than 95%

 

Recipient Percent

0-100%

 

Recipient Result

Detected, Not Detected, Less Than 5%, Detected (Approx %), Greater Than 95%, Insufficient Sample, See Interpretation

TAT

4 days

Additional Notes

Specimen will be rejected if frozen or heparinized bone marrow or peripheral blood

Aliases

Chimerism Study -Sorted Post Sample
Bone Marrow Transplant Engraftment by STR

CPT

CPT code will vary