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Test Code LAB6456 t(15;17) PML/RARA Analysis, Quantitative by RT-PCR

Performing Section

PRECISION DIAGNOSTICS

Purpose/Principle

The PML-RARA One-Step Detection Kit is a multiplex, one-step RT-PCR assay that simultaneously detects the three main PML-RARA t(15;17) translocations commonly associated with Acute Promyelocytic Leukemia (APL): bcr1, bcr2, and bcr3.

Special Collection Notes

Collect specimen according to standard operating procedure.

Specimen Type

Whole Blood/Bone Marrow : Purple Top Tube-EDTA

 

Mouth: Buccal Swab

 

Tissue (Fresh-Frozen, Paraffin Embedded, or RPMI)

Specimen Volume

Whole Blood/Bone Marrow

Preferred: 4.0 mL

Minimum: 0.5 mL

*Receipt of blood or bone marrow within 72 hours of collection. Delays may impact nucleic acid quality.

 

Tissue

Preferred: uncut paraffin-embedded tissue block. Slides: 1 stained and 10 unstained - 10 micron thick slides

*Receipt within 24 hours of cutting or as soon as possible. Delays may impact nucleic acid quality.

Processing Instructions

Store and ship ambient. If transport is longer than 4 hours send refrigerated on cold pack.
For frozen tissue please send on dry ice- ASAP.

Temperature

Ambient/Refrigerated

Test Components

t(15;17) PML/RARA Result
Long (bcr1) CT Replicate 1
Long (bcr1) CT Replicate 2
ABL (Long bcr1) CT Replicate 1
ABL (Long bcr1) CT Replicate 2
LONG (bcr1) Quantity
ABL (Long bcr1) Quantity
LONG (bcr1) NCN Ratio
Short (bcr3) CT Replicate 1
Short (bcr3) CT Replicate 2
ABL (Short bcr3) CT Replicate 1
ABL (Short bcr3) CT Replicate 2
Short (bcr3) Quantity
ABL (Short bcr3) Quantity
Short (bcr3) NCN Ratio
Variant (bcr2) CT Replicate 1
Variant (bcr2) CT Replicate 2
ABL (Variant bcr2) CT Replicate 1
ABL (Variant bcr2) CT Replicate 2

Reference Range

t(15;17) PML/RARA Result

Not Detected

 

*All other components has no reference ranges

TAT

7 days

Additional Notes

Specimen will be rejected if frozen or heparinized bone marrow or peripheral blood

Aliases

PML-RARA
Leukemia by RT-PCR

CPT

81315