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Test Code LAB5891 Herpes Simplex Virus PCR

Performing Section

Microbiology

Purpose/Principle

This real-time PCR assay is intended for qualitative detection and differentiation of HSV-1 and HSV-2 in cutaneous or mucocutaneous lesion swab specimens and cerebrospinal fluid (CSF) samples collected from patients suspected of herpes simplex virus (HSV).

Special Collection Notes

Collect specimen according to standard operating procedure.

Specimen Type

Whole Blood

:

Purple Top- EDTA

 

CSF:

Sterile screw capped container

 

Standard Swab kit:

cutaneious or mucocutaneous lesions

Specimen Volume

Whole Blood

Preferred: 3.0 mL

Minimum: 0.8 mL

 

CSF/Ocular Fluid

Preferred:0.5 mL

Minimum : 0.3 mL

Processing Instructions

Store refrigerated and send on wet ice via courier.
If transport time is greater than 24 hours for CSF, freeze at -20 Celsius and ship on dry ice.
Do not freeze whole blood.

Temperature

Refrigerated/Freeze

Test Components

Herpes Simplex Virus, Type 1
Herpes Simplex Virus, Type 2

Reference Range

Herpes Simplex Virus, Type 1

Not Detected

 

Herpes Simplex Virus, Type 2

Not Detected

TAT

48 Hours

Sample Stability

Refrigerated: 72 hours

Additional Notes

Specimen will be rejected if on the incorrect swab or media.

Aliases

HSV PCR

CPT

87529

Test/Batch Schedule

Monday – Sunday

Method

Diasorin Simplexa