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HelpTest Code LAB5891 Herpes Simplex Virus PCR
Aliases
HSV PCR
Performing Section
Microbiology
Purpose/Principle
This real-time PCR assay is intended for qualitative detection and differentiation of HSV-1 and HSV-2 in cutaneous or mucocutaneous lesion swab specimens and cerebrospinal fluid (CSF) samples collected from patients suspected of herpes simplex virus (HSV).
Special Collection Notes
Collect specimen according to standard operating procedure.
Specimen Type
Whole Blood
:Purple Top- EDTA
CSF:
Sterile screw capped container
Standard Swab kit:
cutaneious or mucocutaneous lesions
Specimen Volume
Whole Blood
Preferred: 3.0 mL
Minimum: 0.8 mL
CSF/Ocular Fluid
Preferred:0.5 mL
Minimum : 0.3 mL
Processing Instructions
Store refrigerated and send on wet ice via courier.
If transport time is greater than 24 hours for CSF, freeze at -20 Celsius and ship on dry ice.
Do not freeze whole blood.
Temperature
Refrigerated/Freeze
TAT
48 Hours
Test Components
Herpes Simplex Virus, Type 1
Herpes Simplex Virus, Type 2
CPT
87529
Additional Notes
Specimen will be rejected if on the incorrect swab or media.
Reference Range
Herpes Simplex Virus, Type 1Not Detected
Herpes Simplex Virus, Type 2
Not Detected
Sample Stability
Refrigerated: 72 hours
Test/Batch Schedule
Monday – Sunday
Method
Diasorin Simplexa