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Test Code LAB1823 Methotrexate Level

Performing Section

Chemistry

Purpose/Principle

Quantitatively measure Methotrexate levels in plasma and serum.

Special Collection Notes

Protect sample from light.

Specimen Type

Serum/Plasma:
Light Green Tube Top-Lithium Heparin (Preferred)
Gold Top Tube-SST
Red Top Tube-Plain

Specimen Volume

Preferred: 0.9 mL (0.3 mL Serum/Plasma)
Minimum: 0.6 mL (0.04 mL Serum/Plasma)

Processing Instructions

CENTRIFUGE sample per procedure.
Store and send sample refrigerated via courier.

Temperature

Refrigerated

Test Components

Hemolysis Index
Icterus Index
Turbidity Index
Methotrexate Level
Methotrexate Level- Diluted (AVG)
Methotrexate Last Dose Start Date
Methotrexate Last Dose Start Time
Methotrexate Last Dose Stop Date
Methotrexate Last Dose Stop Time
MTX Dil 1
MTX Dil 2
MTX Quality Check

Reference Range

Hemolysis Index

This component has no reference ranges

 

Icterus Index

This component has no reference ranges

 

Turbidity Index

This component has no reference ranges

 

Methotrexate Level

>=0.04 mcmol/L

 

Methotrexate Level- Diluted (AVG)

>=0.04 mcmol/L

 

MTX Dil 1

>=0.04 mcmol/L

 

MTX Dil 2

>=0.04 mcmol/L

 

MTX Quality Check

-10.0-10.0 %

TAT

Routine: 120 Minutes
STAT: 75 Minutes

Sample Stability

Ambient: Not recommended
Refrigerated: 14 days
Frozen @-18 Celsius: 8 months (do not exceed 3 freeze/thaw cycle)

Additional Notes

Patients on glucarpidase rescue therapy should be off the drug for 5 to 7 days prior to methotrexate testing. Patients given
glucarpidase may have an increased value for methotrexate.

Aliases

MTX

CPT

80204

Test/Batch Schedule

Runs daily-on demand