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Test Code LAB1823 Methotrexate Level

Aliases

MTX

Performing Section

Chemistry

Purpose/Principle

Quantitatively measure Methotrexate levels in plasma and serum.

Special Collection Notes

Protect sample from light.

Specimen Type

Serum/Plasma:
Light Green Top Tube-Lithium Heparin (Preferred)
Gold Top Tube-SST
Red Top Tube-Plain

Specimen Volume

Preferred: 0.9 mL (0.3 mL Serum/Plasma)
Minimum: 0.6 mL (0.04 mL Serum/Plasma)

Processing Instructions

CENTRIFUGE sample per procedure.
Store and send sample refrigerated via courier.

Temperature

Refrigerated

TAT

Routine: 120 Minutes
STAT: 75 Minutes

Test Components

Hemolysis Index
Icterus Index
Turbidity Index
Methotrexate Level
Methotrexate Level- Diluted (AVG)
Methotrexate Last Dose Start Date
Methotrexate Last Dose Start Time
Methotrexate Last Dose Stop Date
Methotrexate Last Dose Stop Time
MTX Dil 1
MTX Dil 2
MTX Quality Check

CPT

80204

Sample Stability

Ambient: Not recommended
Refrigerated: 14 days
Frozen @-18 Celsius: 8 months (do not exceed 3 freeze/thaw cycle)

Additional Notes

Patients on glucarpidase rescue therapy should be off the drug for 5 to 7 days prior to methotrexate testing. Patients given
glucarpidase may have an increased value for methotrexate.

Test/Batch Schedule

Runs daily-on demand

Reference Range

Hemolysis Index

This component has no reference ranges

 

Icterus Index

This component has no reference ranges

 

Turbidity Index

This component has no reference ranges

 

Methotrexate Level

>=0.04 mcmol/L

 

Methotrexate Level- Diluted (AVG)

>=0.04 mcmol/L

 

MTX Dil 1

>=0.04 mcmol/L

 

MTX Dil 2

>=0.04 mcmol/L

 

MTX Quality Check

-10.0-10.0 %