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HelpTest Code LAB10144 Streptococcus Group A PCR
Aliases
STREPA
BSGA
Performing Section
Microbiology
Specimen Type
Tissue/Aspirate/Fluid/Other Sterile Specimen:
Sterile Container
Swab:
Aerobic Swab (E-Swab)
Specimen Volume
Aspirate/Fluid
Minimum:0.5 mL
Processing Instructions
Store and send ambient ASAP/STAT. Specimen must arrive to the lab within 24 hrs of collection.
TAT
72 hours
CPT
87651
Special Collection Notes
Collect specimen according to standard operating procedure.
Tissues, Aspirates, Fluids, and other Sterile Specimens: Collect specimen aseptically into a sterile specimen container.
Collect an aerobic culture swab using a COPAN ESwab. DO NOT collect with MRSA swab. Throat swabs are NOT acceptable.
Method
ABI 7500
Purpose/Principle
In this assay, DNA extracted from patient samples is subjected to real-time PCR amplification using
primers and a fluorescent probe directed against a target sequence in the Strep A genome (spy1258) (Kodani, et al 2011). The product is detected on an Applied Biosystems 7500 Taqman™ instrument using 5’-nuclease methodology. An exogenous positive control is co-amplified in each reaction to determine extraction efficiency and detect inhibition. Results are reported as Strep A Detected or Not Detected.
Temperature
Ambient
Sample Stability
Ambient: 24 hours
Test/Batch Schedule
Monday – Sunday
Test Components
Streptococcus Group A Qual
Reference Range
Streptococcus Group A QualNot Detected