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HelpTest Code LAB10055 Proinflammatory Cytokines
Aliases
IL-1beta, IL-2, IL-6, TNF-alpha
Performing Section
Immunology/Flow
Specimen Type
Plasma:
Purple Top- EDTA
Specimen Volume
Whole Blood:
Preferred: 1.5 mL
Minimum: 0.6 mL
Processing Instructions
1) Centrifuge sample to separate plasma per procedure. Plasma must be separated within 1 hour of collection.
2) Pipette off plasma and split into two equal aliquots unless less than 0.2 mL total, then put into 1 aliquot.
3) Place sample in freezer (preferably immediately, or within 30 minutes) until courier arrives.
4) Send sample frozen (on dry ice) via courier.
TAT
96 hours
CPT
83520×4
Special Collection Notes
Ensure that samples collected in red top tubes have been fully clotted and separated.
Additional Notes
Grossly hemolyzed or lipemic samples are not suitable for testing, such samples will not be run and will be rejected.
Method
Enzyme-Linked Immunosorbent Assay (ELISA)
Purpose/Principle
This method uses a microfluidics based immunoassay performed inside a consumable microfluidic Simple Plex assay cartridge. Similar to a pre-kitted ELISA, analytes are pre-loaded into the cartridge and wash buffer and patient samples are added into the cartridge and loaded onto the Ella instrument. Inside each fluidic channel are three glass nanoreactors (GNRs) coated with capture antibody, providing built-in replicate measurements for each analyte. With pre-loaded factory calibrated standard curves, fully analyzed data is available in less than 90 minutes.
Temperature
Frozen
Sample Stability
Frozen at -80 Celsius: 2 years
Test/Batch Schedule
Tues & Friday
Test Components
Interleukin 1 beta
Interleukin 2
Interleukin 6
Tumor necrosis factor alpha
Reference Range
Interleukin 1 beta0 up to 2 years: <4.8 pg/mL
2 years up to 7 years: <6.5pg/mL
7 years up to 18 years: <17.6 pg/mL
18 years and up: <7.4 pg/mL
Interleukin 2
0 up to 2 years: <5.3 pg/mL
2 years up to 7 years: <7.4 pg/mL
7 years up to 18 years: <7.1 pg/mL
18 years and up: <9.2 pg/mL
Interleukin 6
0 up to 2 years: <18.7 pg/mL
2 years up to 7 years: <22.1 pg/mL
7 years up to 18 years: <42.4 pg/mL
18 years and up: <13.1 pg/mL
Tumor necrosis factor alpha
0 up to 2 years: <20.5 pg/mL
2 years up to 7 years: <20.5 pg/mL
7 years up to 18 years: <14.1 pg/mL
18 years and up: <10.0 pg/mL