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Test Code LAB10055 Proinflammatory Cytokines

Performing Section

Immunology/Flow

Purpose/Principle

This method uses a microfluidics based immunoassay performed inside a consumable microfluidic Simple Plex assay cartridge. Similar to a pre-kitted ELISA, analytes are pre-loaded into the cartridge and wash buffer and patient samples are added into the cartridge and loaded onto the Ella instrument. Inside each fluidic channel are three glass nanoreactors (GNRs) coated with capture antibody, providing built-in replicate measurements for each analyte. With pre-loaded factory calibrated standard curves, fully analyzed data is available in less than 90 minutes.

Special Collection Notes

Ensure that samples collected in red top tubes have been fully clotted and separated.

Specimen Type

Plasma/Serum:
Purple Top- EDTA (Preferred)
Red Top- Plain

Specimen Volume

Whole Blood:
Preferred: 1.5 mL
Minimum: 0.6 mL

Processing Instructions

1) CENTRIFUGE sample per procedure.
2) Pipette off plasma or serum and split into two equal aliquots unless less than 0.2 mL total, then put into 1 aliquot.
3) Attach original tube to pour off tubes with rubber band.
4) Place sample in freezer until courier arrives.
5) Send sample Frozen (on dry ice) via courier.

Temperature

Frozen

Test Components

Interleukin 1 beta
Interleukin 2
Interleukin 6
Tumor necrosis factor alpha

Reference Range

Interleukin 1 beta

0 up to 2 years: <4.8 pg/mL

2 years up to 7 years: <6.5pg/mL

7 years up to 18 years: <17.6 pg/mL

18 years and up: <7.4 pg/mL

 

Interleukin 2

0 up to 2 years: <5.3 pg/mL

2 years up to 7 years: <7.4 pg/mL

7 years up to 18 years: <7.1 pg/mL

18 years and up: <9.2 pg/mL

 

Interleukin 6

0 up to 2 years: <18.7 pg/mL

2 years up to 7 years: <22.1 pg/mL

7 years up to 18 years: <42.4 pg/mL

18 years and up: <13.1 pg/mL

 

Tumor necrosis factor alpha

0 up to 2 years: <20.5 pg/mL

2 years up to 7 years: <20.5 pg/mL

7 years up to 18 years: <14.1 pg/mL

18 years and up: <10.0 pg/mL

TAT

96 hours

Sample Stability

Frozen at -80 Celsius: 2 years

Additional Notes

Grossly hemolyzed or lipemic samples are not suitable for testing, such samples will not be run and will be rejected.

Aliases

IL-1beta, IL-2, IL-6, TNF-alpha

CPT

83520×4

Test/Batch Schedule

Monday-Thursday

Method

Enzyme-Linked Immunosorbent Assay (ELISA)