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HelpTest Code LAB10040 Enterovirus and Parechovirus Multiplex PCR
Performing Section
Microbiology
Purpose/Principle
Due to a need to distinguish enterovirus from parechovirus infection, and to specifically detect EV-D68 and EV-A71, Children’s Hospital Colorado developed an in-house assay for detection of enterovirus, parechovirus, EV-D68, and EV-A71 RNA from upper respiratory, lesion, throat, and colorectal swabs, and plasma. In this assay, RNA extracted from patient samples is subjected to reverse transcription followed by realtime PCR amplification using primers and fluorescent probes targeted against the four components (panenterovirus, pan-parechovirus, EV-D68, and EV-A71).
Special Collection Notes
Collect specimen per standard operating procedure.
If transport requires greater than two hours from patient to laboratory, place stool in a Cary-Blair Media container.
Specimen Type
Throat/Rectal/Surface Lesion Swab: Standard Swab with UTM/VTM MediaWhole Blood: Purple Top Tube-EDTA
Specimen Volume
Preferred: 4 mL
Minimum: 1.0 mL
Processing Instructions
DO NOT CENTRIFUGE
Store refrigerated and Ship on wet ice.
Temperature
Refrigerated
Test Components
Parechovirus PCR Qual
Enterovirus PCR Qual
Enterovirus D-68 PCR
Enterovirus A71
Reference Range
Parechovirus PCR QualNot Detected
Enterovirus PCR Qual
Not Detected
Enterovirus D-68 PCR
Not Detected
Enterovirus A71
Not Detected
TAT
72 Hours
Additional Notes
Unacceptable specimens:
Multisource swabs, unlabeled, mislabeled, or comingled specimens. For sample types/sources not listed, medical or scientific director approval is required.
Aliases
EV/PeV PCR
EV PCR
PEHV PCR
Echovirus
Coxsackie Virus
CPT
87498×3